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VANTAstar

O novo VANTAstarTM é o mais novo membro de nossa família. Este leitor de microplacas multimodo compacto foi concebido para ser operado de forma fácil e flexível assegurando que a programação do equipamento seja feita sem esforço para geração de dados de forma simplificada. Equipado com nosso sistema patenteado de monocromadores duplos LVF, filtros, espectrômetro UV/VIS e tecnologia de faixa dinâmica estendida EDR, o VANTAstarTM pode atender uma ampla gama de aplicações em pesquisa básica e ciências da vida.

VANTAstar

O VANTAstar é um leitor de microplacas multimodo configurável compatível com todos os modelos de placas até 384 poços. A tecnologia de faixa dinâmica estendida (EDR) combinada com o sistema de autofoco aliados a redução de interferências cruzadas em luminescência faz com que os ensaios sejam realizados de maneira simples e sem otimizações manuais supérfluas.

Com a Unidade de Controle Atmosférico, incubação, múltiplas opções de agitação, escaneamento de poços e injetores de reagentes com aquecedor e misturador, o VANTAstar atende à todos os ensaios com base em células vivas.
Monocromadores LVF para flexibilidade e performance para varredura

VANTAstar

  • Detection Modes

    Fluorescence intensity (incl. FRET)

    Fluorescence polarization

    Luminescence (flash and glow) – incl. BRET

    Time-resolved fluorescence

    TR-FRET

    UV/vis absorbance spectra

  • Measurement Modes

    Top and bottom reading

    Endpoint and kinetic

    Sequential multi-excitation

    Sequential multi-emission

    Spectral scanning (fluorescence, luminescence, absorbance)

    Ratiometric measurements

    Well scanning

  • Microplate Formats

    6- to 384-well plates, user-definable

    LVis Plate with 16 low volume microspots (2 μL)

  • Microplate Carrier

    Robot compatible

  • Light Sources

    High energy xenon flash lamp

  • Detectors

    Low-noise photomultiplier tube

    CCD spectrometer

  • Wavelength Selection

    Dual Linear Variable Filter (LVF) Monochromators™

    Linear Variable Dichroic Mirror

    Separates ex & em LVF Monochromators

    Optical filters:

    Ex and em slides hold up to 5 filters each

    LVF Monochromators + optical filters

    Use one for ex and the other for em

    UV/Vis absorbance spectrometer:

    Full spectra or 8 distinct wavelengths in < 1 sec/well

  • Optical Filters

    Excitation and emission slides for up to 4 filters each for monochoromator units (VANTAstar), and for up to 5 filters each for filter-only units (VANTAstar F)

  • Optical Path Guides

    Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics

  • Z-Adjustment

    Automatic focal height adjustment (0.1 mm resolution)

  • Spectral Range
    Filters FI, LUM, TRF, FP, TR-FRET: 240 – 740 nm
    LVF Monochromators™ FI, LUM: 320 – 740 nm
    Linear Variable Dichroic 340 – 740 nm
    Spectrometer ABS: 220 – 1000 nm; wavelength precision: ≤ 0.5 nm
  • Sensitivity
    FI filters
    (top)
    < 0.5 pM (< 10 amol/well FITC, 384sv, 20 μL)
    FI filters
    (bottom)
    < 2.5 pM (< 125 amol/well FITC, 384g, 50 μL)
    FI monochromator
    (top)
    < 0.8 pM (< 16 amol/well FITC, 384sv, 20 μL)
    FI monochromator
    (bottom)
    < 3.5 pM (< 175 amol/well FITC, 384g, 50 μL)

    Dynamic Range: 8 decades in a single measurement

    FP < 1.2 mP SD at 1 nM FITC (384sv, 20 μL)
    HTRF®
    (black and white microplates)
    Reader Control Kit (Eu) after 18h (384sv, 20 μL)
    Delta F > 700% (High Calibrator)
    Delta F > 25% (Low Calibrator)
    TRF < 30 fM europium (< 2.4 amol/well, 384, 80 μL)
    LUM < 0.8 pM (< 15 amol/well ATP, 384sv, 20 μL)
    Dynamic range: 8 decades in a single measurement
    ABS
    with spectrometer
    Selectable spectral resolution: 1, 2, 5, and 10 nm
    OD range: 0 to 4 OD; photometric resolution: 0.001 OD
    Accuracy: < 1% at 2 OD
    Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
    Linearity: ≤ 0.8% at 2.0 OD
  • Read Times
    Flying mode (1 flash) 9 sec (96), 16 sec (384)
  • Reagent Injection

    Up to 2 reagent injectors
    Individual injection volumes for each well: 3 to 500 μL (optionally up to 1 mL)
    Variable injection speed up to 420 μL/s
    Reagent back flushing
    Heater and stirrer plate
    Dimensions: width: 13 cm, depth: 31 cm, height: 26 cm; weight: 6 kg

  • Shaking

    Linear, circular and double-circular with user-definable time and speed

  • Incubation

    +4°C above ambient up to 45°C or 60°C

    Minimum condensation concept: the upper heating plate of the incubation chamber operates at +0.5°C than the lower plate, heated optic system.

  • Software

    Multi-user Smart Control and MARS Data Analysis Software included
    FDA 21 CFR Part 11 compliant

    Integrated fluorophore library

  • Dimensions

    Width: 35 cm, depth: 52 cm, height: 36 cm; weight: 27 kg