Microplate reader solutions for Agilent’s Cell Metabolism Assays 28 de abril de 2020       Nenhum comentário Plate reader solutions for measuring cellular metabolism   Agilent brochure           Leia Mais Enhanced degradation of the antibiotic sulfamethoxazole by heterogeneous photocatalysis using Ce0,8Gd0,2O2-d/TiO2 particles 19 de setembro de 2019       Nenhum comentário Clique aqui para ler o artigo Leia Mais Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlokTM and FLUOstar OPTIMA 17 de julho de 2019       Nenhum comentário Introduction The mechanism of prostate metastasis to bone marrow is a complex multistage process that is only beginning to be understood. Initial steps include down regulation of molecular binding complexes and productio... Leia Mais ADP HunterTM Assay for HTS of Kinase Inhibitors using the PHERAstar 17 de julho de 2019       Nenhum comentário Introduction As mediators of eukaryotic signal transduction, controlling multiple cellular processes such as gene transcription, cell cycling, migration, apoptosis and differentiation, protein kinases are considered impo... Leia Mais Promega’s Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar 17 de julho de 2019       Nenhum comentário Introduction Today’s high-throughput screening facilities face increasing demands to generate more information from their existing compound libraries. One method of obtaining this information is to run assays sequentia... Leia Mais High-Throughput Measurement of Protein Stability using a FLUOstar OPTIMA Microplate Reader 17 de julho de 2019       Nenhum comentário Introduction The measurement of protein stability is essential for elucidating protein function in vivo, engineering the improved stability of therapeutic proteins or biocatalytic enzymes, and formulating proteins for th... Leia Mais Rapid Functional Screening of Dopamine Receptors by Measuring Intracellular Calcium with the NOVOstar Fluorescence Reader 17 de julho de 2019       Nenhum comentário Introduction Calcium is a common second messenger released upon stimulation of different G-protein coupled receptors (GPCRs). Measuring intracellular calcium is therefore a widely applicable method for functional screeni... Leia Mais Fluorescence Analysis of Reactive Oxygen Species (ROS) Generated by Six Isolates of Aspergillus fumigatus 17 de julho de 2019       Nenhum comentário Introduction Reactive oxygen species (ROS) are essential intermediates in oxidative metabolism. Nonetheless, when generated in excess, ROS can damage cells by peroxidizing lipids and disrupting structural proteins, enzym... Leia Mais Intracellular Calcium Assay on NOVOstar Microplate Reader 17 de julho de 2019       Nenhum comentário Introduction Many biological signals are transmitted by increasing or decreasing the intracellular calcium concentration (receptors, ion channels). Intracellular calcium assays are therefore used for studying a great num... Leia Mais Molecular Probes PicoGreen® Assay Performed on BMG LABTECH POLARstar OPTIMA Microplate Reader 17 de julho de 2019       Nenhum comentário Introduction State of the art biotechnology relies on many different analytical techniques in combination with high throughput screening (HTS) methodology. The detection and quantitation of minute amounts of DNA is routi... Leia Mais